Chromatin is of central importance to gene regulation in eukaryotes. Reflecting this singular role for chromatin, numerous approaches have evolved in the laboratory over the past three decades to study chromatin structure and its alterations. Methods of investigating chromatin remodeling, whether in changes in nucleosome structure or position with respect to the incorporated DNA or in histone modifications, have progressed rapidly over the recent years. In Chromatin Remodeling: Methods and Protocols , expert researchers contribute chapters which include methods for investigating chromatin remodeling in vitro and in vivo, in yeast, plants, and mammalian cells, and at local and global levels.
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Fifty Shades of Grey Gets BDSM Dangerously Wrong - The Atlantic
The blockbuster fantasy has become a big movie—and a bigger problem. What is a fantasy? From Freud to Ludacris , it's been an elusive idea, suggesting both an escape from reality and an expression of hidden desire. In culture, fantasy works like a mirror: It reflects who we are, but it also shapes what we become. Love it or despise it, American culture's sexual fantasy of the moment is Fifty Shades of Grey.
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